Molecular Biology Intern (High-Throughput Assays)

<p><strong>About the Role:</strong></p> <p>The intern will join the spatial proteomics R&amp;D effort, focusing on building and optimizing high-throughput experimental workflows for screening custom proteins. They will work with display systems (e.g., yeast display) and surface-based assays to evaluate affinity, specificity, and performance of designed proteins across multiple formats.</p> <p>On a day-to-day basis, the intern will design and clone constructs, develop and run high-throughput binding assays, establish and optimize conjugation strategies to link proteins to DNA or other reporters, and analyze large datasets from screening experiments to identify the most promising candidates. They will work closely with a cross-functional team spanning molecular biology, protein engineering, and computational design, and will regularly share results and recommendations with the broader program.</p> <p><strong>What you Will Be Doing:</strong></p> <p><span style="font-size: 10pt;"><strong>Assay &amp; Platform Development</strong></span></p> <ul> <li>Develop, optimize, and execute high-throughput screening assays to evaluate custom proteins.</li> <li>Build and refine yeast surface display workflows (and/or related display platforms) for quantitative binder characterization using flow cytometry.</li> <li>Establish and compare surface-based screening formats, such as:</li> <ul> <li>Yeast display + FACS</li> <li>Surface-immobilized target assays (e.g., beads, chips, slides)</li> <li>Plate-based or bead-based binding and competition assays.</li> </ul> <li>Implement and benchmark conjugation strategies that link proteins to DNA or other reporter molecules for multiplexed assays and barcoded readouts.</li> </ul> <p><span style="font-size: 10pt;"><strong>Molecular Biology &amp; Protein Engineering</strong></span></p> <ul> <li>Design and execute cloning strategies for custom protein libraries and individual variants (PCR, Gibson/Golden Gate, site-directed mutagenesis).</li> <li>Construct and validate expression vectors for yeast and/or other systems, including QC of expression and display levels.</li> <li>Support expression and basic QC of selected binders (e.g., SDS-PAGE, Western blot, simple purification or lysate-based functional assays).</li> </ul> <p><span style="font-size: 10pt;"><strong>High-Throughput Screening &amp; Data Analysis</strong></span></p> <ul> <li>Plan and perform FACS-based and/or plate-based binder screening experiments, including appropriate controls, titrations, and replicates.</li> <li>Analyze high-dimensional assay data (e.g., from flow cytometry or barcoded readouts) to rank candidates based on affinity, specificity, and other performance metrics.</li> <li>Where applicable, prepare samples and data for sequencing-based readouts and collaborate with others to interpret sequence–function relationships.</li> <li>Summarize findings in clear figures, tables, and internal reports, and present progress in regular team meetings.</li> </ul> <p><span style="font-size: 10pt;"><strong>Collaboration &amp; Documentation</strong></span></p> <ul> <li>Work with computational design, protein production, and assay development teams to integrate experimental results into the design–build–test–learn cycle.</li> <li>Maintain detailed electronic lab notebook records, SOPs, and reagent documentation to ensure reproducibility and handoff.</li> <li>Contribute to a final internal report and presentation describing the developed workflows, experimental results, and recommendations for future development.</li> </ul> <p><strong>To Be Successful You Will Need:</strong></p> <ul> <li>Current PhD candidate in Biochemistry, Biophysics, Molecular Biology, Bioengineering, Chemical Biology, or a closely related field.</li> <li>Ideally within the final 1–2 years of their PhD program, with substantial hands-on experimental training in protein or molecular biology.</li> <li>Proven ability to independently design, execute, and troubleshoot experiments.</li> <li>Strong quantitative and analytical mindset, including comfort with large datasets from high-throughput screens.</li> <li>Clear written and verbal communication skills, with the ability to present complex results across disciplines.</li> <li>Highly organized, collaborative, and comfortable working in a fast-paced, interdisciplinary R&amp;D environment.</li> </ul> <p><strong>Core Technical Skills (Required):</strong></p> <ul> <li>Strong molecular biology foundation:</li> <ul> <li>PCR, cloning (e.g., Gibson, Golden Gate), site-directed mutagenesis.</li> <li>Plasmid design, sequence verification, and basic construct troubleshooting.</li> </ul> <li>Experience with protein expression and characterization in at least one system (yeast, E. coli, or cell-free):</li> <ul> <li>Induction/optimization of expression, basic purification or lysate-based assays, SDS-PAGE/Western.</li> </ul> <li>Hands-on experience with high-throughput or quantitative assay formats, such as:</li> <ul> <li>Flow cytometry / FACS, plate-based binding assays (ELISA or similar), or cell-based functional assays.</li> </ul> <li>Comfort with data analysis and visualization:</li> <ul> <li>Experience with Python, R, or analysis tools such as GraphPad Prism is nice to have.</li> <li>General awareness and experience with AI data analysis</li> </ul> </ul> <p><strong>Preferred Experience (Nice to Have):</strong></p> <ul> <li>Yeast surface display, phage display, ribosome/mRNA display, or other library-based screening technologies.</li> <li>Experience with <strong>conjugation and bioconjugation methods</strong>, for example:</li> <ul> <li>Cysteine-based conjugation (maleimide-thiol, halo tag, SNAP/CLIP, etc.).</li> <li>Click chemistry (e.g., azide–alkyne, DBCO, strain-promoted cycloaddition).</li> <li>DNA-barcoding of proteins, or other methods linking proteins to nucleic acids or reporters.</li> </ul> <li>Familiarity with <strong>translation-linked or puromycin-based</strong> conjugation methods, or related approaches that couple protein synthesis to covalent capture is highly desired.</li> <li>Background in protein engineering, antibody/nanobody development, or de novo mini-binder workflows.</li> </ul> <p>&nbsp;</p><div class="content-pay-transparency"><div class="pay-input"><div class="description"><p>Below is the base pay range for this position.&nbsp; The actual base pay will depend on several factors unique to each candidate, including one’s skills, qualifications, and experience.&nbsp; Unless otherwise required by law, this role is not eligible for 10x’s health benefit program.&nbsp; During the hiring process, your 10x recruiter can share more information about the Company’s compensation and benefits.</p></div><div class="title">Pay Range</div><div class="pay-range"><span>$40</span><span class="divider">&mdash;</span><span>$48 USD</span></div></div></div><div class="content-conclusion"><p><strong>About 10x Genomics</strong></p> <p>At 10x Genomics, accelerating our understanding of biology is more than a mission for us. It is a commitment. This is the century of biology, and the breakthroughs we make now have the potential to change the world.<br><br>We enable scientists to advance their research, allowing them to address scientific questions they did not even know they could ask. Our tools have enabled fundamental discoveries across biology including cancer, immunology, and neuroscience.<br><br>Our teams are empowered and encouraged to follow their passions, pursue new ideas, and perform at their best in an inclusive and dynamic environment. We know that behind every scientific breakthrough, there is a deep infrastructure of talented people driving the life sciences industry and making it possible for scientists and clinicians to make new strides. We are dedicated to finding the very best person for every aspect of our work because the innovations and discoveries that we enable together will lead to better technologies, better treatments, and a better future. Find out how you can make a 10x difference.&nbsp;</p> <p><em>Individuals seeking employment at 10x Genomics are considered without regards to race, color, religion, national origin, age, sex, marital status, ancestry, physical or mental disability, veteran status, gender identity, or sexual orientation,&nbsp;or any other characteristic protected by applicable law.</em></p> <p>10x does not accept unsolicited applicants submitted by third-party recruiters or agencies. Any resume or application submitted to 10x without a vendor agreement in place will be considered unsolicited and property of 10x, and 10x will not pay a placement fee.</p> <p><a href="https://www.linkedin.com/company/10xgenomics/" target="_blank"><img src="https://pngmind.com/wp-content/uploads/2019/08/Linkedin-Logo-Png-Transparent-Background.png" alt="LI Image" width="40" style="max-width: 100%;"></a> &nbsp;&nbsp;&nbsp;<a href="https://twitter.com/10xgenomics" target="_blank"><img src="https://www.freepnglogos.com/uploads/twitter-logo-png/twitter-logo-vector-png-clipart-1.png" alt="Twitter Icon" width="40" style="max-width: 100%;"></a></p></div>